525. Directed Molecular Evolution of Transcription Factors in Mammalian Cells for Enhanced Directed Cell Differentiation

Directed Differentiation of Embryonic Stem Cells Into Cardiomyocytes by Bacterial Injection of Defined Transcription Factors

Forced expression of defined transcriptional factors has been well documented as an effective method for cellular reprogramming or directed differentiation. However, transgene expression is not amenable for therapeutic application due to potential insertional mutagenesis. Here, we have developed a bacterial type III secretion system (T3SS)-based protein delivery tool and shown its application i...

Directed Molecular Evolution – Applications in Biocatalysis

drug discovery are announced on a regular basis, it is rare for an entirely new technology to emerge within the pharmaceutical bulk active and synthesis sector. Such a technology – developed by Codexis, Inc – enables the rapid discovery and development of novel or improved processes for the cost-effective synthesis of chemicals, using breakthrough biocatalysts and fermentation strains. Furtherm...

Directed differentiation of progenitor cells towards an islet-cell phenotype.

Exogenous insulin administration and oral anti-diabetic drugs are the primary means of treating diabetes. However, tight glycaemic control, with its inherent risk of hypoglycaemia, is required to prevent the microvascular and macrovascular complications of the disease. While islet or pancreas transplantations offer a longer-term cure, their widespread application is not possible, primarily beca...

Directed molecular evolution of ADP-glucose pyrophosphorylase.

ADP-glucose pyrophosphorylase catalyzes a rate-limiting reaction in prokaryotic glycogen and plant starch biosynthesis. Despite sharing similar molecular size and catalytic and allosteric regulatory properties, the prokaryotic and higher plant enzymes differ in higher-order protein structure. The bacterial enzyme is encoded by a single gene whose product of ca. 50,000 Da assembles into a homote...

SnapShot: Directed Differentiation of Pluripotent Stem Cells